Culturing a Sputum Specimen for Mycobacteria
| Purpose | To study mycobacteria in culture |
| Materials | Lowenstein-Jensen slants Simulated sputum culture (predigested and concentrated) |
Procedures
- Prepare an acid-fast stain directly from the sputum specimen (review Exercise 6). Read and record observations.
- Inoculate the specimen on Lowenstein-Jensen medium and incubate at 35°C until growth appears.
- Examine for visible growth and record appearance.
Results
- Diagram observations of the stained smear.
- Describe the growth on Lowenstein-Jensen medium. How many days before growth appeared?
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