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  Section: General Biotechnology / Genes & Genetic Engineering
 
 
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Genes : Nature, Concept and Synthesis

 
     
 

Structure of Ribonucleic Acid (RNA)
The RNA is usually single stranded except some viruses such as TMV, yellow mosaic virus, influenza virus, foot and mouth disease virus, reovirus, wound tumour virus, etc. which have dsDNA. The single strand of the RNA is folded either at certain regions or entirely to form hairpin shaped structure. In the hairpin shaped structures the complementary bases are linked by hydrogen bonds which give stability to the molecules. However, no complementary bases are found in the unfolded region. The RNA does not possess equal purine-pyrimidine ratio, as it is found in the DNA.

Like DNA, the RNA is also the polymer of four nucleotides—each one contains D-ribose, phosphoric acid and a nitrogenous base. The bases are two purines (A,G) and two pyrimidines (C, U). Thyamine is not found in RNA. Pairing between bases occurs as A-U and G-C. The nucleotides formed by the four bases are adenosine monophosphate. (AMP), guanosine monophosphate (GMP), cytosine monophosphate (CMP), and uridine monophosphate (UMP). These are found freely in nucleoplasm but in the form of triphosphates e.g. ATP, GTP, UTP and CTP. As a result of polymerization the ribonucleotides form a polynucleotide chain of RNA.

If the RNA is involved in genetic mechanism, it is called genetic RNA as found in r)lant, animal and bacterial viruses. The DNA acts as genetic material and RNA follows the order of DNA. In such cells the RNA does not have genetic role. Therefore, it is called non-genetic RNA. The non-genetic RNA is of three types : (i) ribosomal RNA (rRNA), (ii) transfer RNA (tRNA) or soluble RNA (sRNA), and (iii) messenger RNA (mRNA) or template RNA. These three types of RNA differ from each other in structure, site of synthesis in eukaryotic cell and function.

The mRNA and tRNA are synthesized on DNA template, whereas rRNA is delivered from nucleolar DNA. These RNAs are synthesized during different stages. During cleavage most of mRNA is synthesized , whereas tRNA is synthesized at the end of cleavage. Synthesis of rRNA occurs during gastrulation. The total population of rRNA is about 90% of all RNAs.

 

Content

Chemical nature of DNA

 

Chemical composition

 

Nucleotides, nucleosides

 

Polynucleotides

 

Chargaff's rule of equivalence

Physical nature of DNA

 

Watson and Cricks model of DNA

 

Circular and superhelical DNA

 

Organization of DNA in eukaryotes

Structure of RNA

Gene concept

Units of a gene

 

Cistron

 

Recon

 

Mutan

Split genes (introns)

 

RNA splicing

 

Ribozyme

 

Evolution of split genes

Overlapping gene

Gene organization

Gene expression

Gene regulation

 

Transcription

 

 

The lac operon (structural gene, operator gene, promoter gene and repressor gene)

Artificial synthesis of genes

 

Synthesis of a gene for yeast alanine tRNA

 

Synthesis of a gene for bacterial tyrosine tRNA

 

Synthesis of a human leukocyte interferon gene

Gene synthesis by using mRNA

Gene machine

The PCR

 

Amplification of DNA (melting of target DNA, annealing of primers, primer extension)

 

Application of PCR technology



Like DNA, the RNA is not self-replicating but it has to depend on DNA. Therefore, replication of non-genetic RNA is known as DNA dependent RNA replication. Moreover, the genetic RNA of viruses is self-replicating i.e. it can form its own several replica copies. Differences between the DNA and RNA molecules are given in Table 2.3.


Table 2.3. Differences between RNA and DNA.

 

RNA

 

DNA

1.

RNA is more primitive than DNA

1.

DNA originated after RNA

 

 

 

 

2.

RNA is the genetic material of some

plant, animal and bacterial viruses.

2.

DNA is the genetic material of almost all living organisms

 

 

 

 

3.

Except some viruses (e.g. reovirus) most cellular RNA is single stranded.

3.

Except a few viruses (e.g. ΦX174), most DNA is double stranded.

 

 

 

 

4.

Pentose sugar is ribose.

4.

Pentose sugar is deoxyribose.

 

 

 

 

5.

The bases are adenine, guanine, cytosine and uracil.

5.

The bases are adenine, guanine, cytosine and thymine.

 

 

 

 

6.

Base pairing occurs between adenine and uracil (A-U), and guanine and cytosine (G-C).

6.

Base pairs are A-T and G-C.

 

 

 

 

7.

Base pairing is seen only in hairpin
structure and helical region.

7.

Base pairing occurs throughout the length
of DNA molecule.

 

 

 

 

8.

RNA contains a few (about 12,000)
nucleotides

8.

DNA contains millions of nucleotides
e.g. over 4 millions.

 

 

 

 

9.

The RNA molecules are of three types: rRNA, mRNA, tRNA.

9.

DNA is only of one type

 

 

 

 

10.

The mRNA is found in nucleolus, tRNA and rRNA are found in cytoplasm. They are formed on the DNA.

10.

DNA is found in chromosomes.

DNA is also found in mitochondria and chloroplasts.

 

 

 

 

11.

RNAs translate the transcripts of DNA
into proteins.

11.

DNA encodes the genetic masses in a
forms the transcripts.

 

 

 

 

12.

Genetic RNA uses the enzyme reverse
transcriptase during replication.

12.

This enzyme is not required by DNA.
DNA after replication forms DNA and
after transcription forms RNA.

 
     
 
 
     



     
 
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