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  Section: General Biotechnology / Genes & Genetic Engineering
 
 
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Genes : Nature, Concept and Synthesis

 
     
 

Units of a Gene
After much extensive work done by the molecular biologists the nature of gene became clear. A gene can be defined as a polynucleotide chain that consists of segments each controlling a particular trait. Now, genes are considered as a unit of function (cistron), a unit of mutation (mutan) and a unit of recombination (recon).

Cistron
One-gene-one enzyme hypothesis of Beadle and Tatum was redefined by several workers in coming years. A single mRNA is transcribed by a single gene. Therefore, one-gene-one mRNA hypothesis was put forth. Exceptionally, a single mRNA is also transcribed by more than one gene and it is said to be polycistronic. Therefore, the concept has been given as one-gene-one protein hypothesis. The proteins are the polypeptide chain of amino acids translated by mRNA. Therefore, it has been correctly used as one-gene-one polypeptide hypothesis.

Moreover, genes are present within the genes and their cis-trans effect govern the function. Therefore, S. Benzer termed the functional gene as cistron (Fig. 2.6A). Crossing over within the functional genes or cistron is possible. The cis and trans arrangement of alleles may be written as below:


Recon
Earlier, it was thought that crossing over occurs between two genes. In 1962, Benzer demonstrated that the crossing over or recombination occurs within a functional gene or cistron. In a cistron the recombinational units may be more than one. Thus, the smallest unit capable of undergoing recombination is known as recon (Fig. 2.6B).

Benzer (1955) found that the cultures of T4 bacteriophage formed plaques on agar plates of Eshcherchia coli. Normal T4 formed small plaques of smooth edges, whereas die mutant T4 phagc formed the larger plaques of rough edges. The DNA molecule of T4 phage consists of several genes one of which is called rll region. Formation of rough edged plaques was governed by two adjacent genes (cistrons rIIA and rIIB) in mutant bacteriophage. Both the regions function independently and consist of 2,500 and 1,500 nucleotides, respectively. In rIIA gene over 500 mutational sites are present where crossing over may occur. Through crossing over exchange of two segments of DNA occurs. If crossing over takes place within the gene, by mating two rII mutant of T4 phage a normal wild type phage can be produced. Thus, the work of Benzer lends support that crossing over within a gene occurs, which explains that the recombinational unit (recon) is much less smaller than the functional unit i.e. cistron.

 

Content

Chemical nature of DNA

 

Chemical composition

 

Nucleotides, nucleosides

 

Polynucleotides

 

Chargaff's rule of equivalence

Physical nature of DNA

 

Watson and Cricks model of DNA

 

Circular and superhelical DNA

 

Organization of DNA in eukaryotes

Structure of RNA

Gene concept

Units of a gene

 

Cistron

 

Recon

 

Mutan

Split genes (introns)

 

RNA splicing

 

Ribozyme

 

Evolution of split genes

Overlapping gene

Gene organization

Gene expression

Gene regulation

 

Transcription

 

 

The lac operon (structural gene, operator gene, promoter gene and repressor gene)

Artificial synthesis of genes

 

Synthesis of a gene for yeast alanine tRNA

 

Synthesis of a gene for bacterial tyrosine tRNA

 

Synthesis of a human leukocyte interferon gene

Gene synthesis by using mRNA

Gene machine

The PCR

 

Amplification of DNA (melting of target DNA, annealing of primers, primer extension)

 

Application of PCR technology




Muton

Benzer (1962) coined the term mutan to denote the smallest unit of chromosome that undergoes mutational changes. Hence, mutan may be defined as 'the smallest unit of DNA which may be changed in the nudeotide. Thus, changes at nucleotide level are possible (Fig. 2.6C). The smallest unit of mutan is the nucleotide. Therefore, cistron is largest unit in size followed by recon and mutan. This can be explained that a gene consists of several cistron, a cistron contains many recon, and a recon a number of mutans. However, if the size of a recon is equal to mutan, there would be no possibility in recon for consisting of several mutans.
  The genes as a unit of function i.e. cistron (A), recombination i.e. recon (B) and mutation i.e. muton (C)
   

Fig. 2.6. The genes as a unit of function i.e. cistron (A), recombination i.e. recon (B) and mutation i.e. muton (C)

 
     
 
 
     



     
 
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