Algae, Tree, Herbs, Bush, Shrub, Grasses, Vines, Fern, Moss, Spermatophyta, Bryophyta, Fern Ally, Flower, Photosynthesis, Eukaryote, Prokaryote, carbohydrate, vitamins, amino acids, botany, lipids, proteins, cell, cell wall, biotechnology, metabolities, enzymes, agriculture, horticulture, agronomy, bryology, plaleobotany, phytochemistry, enthnobotany, anatomy, ecology, plant breeding, ecology, genetics, chlorophyll, chloroplast, gymnosperms, sporophytes, spores, seed, pollination, pollen, agriculture, horticulture, taxanomy, fungi, molecular biology, biochemistry, bioinfomatics, microbiology, fertilizers, insecticides, pesticides, herbicides, plant growth regulators, medicinal plants, herbal medicines, chemistry, cytogenetics, bryology, ethnobotany, plant pathology, methodolgy, research institutes, scientific journals, companies, farmer, scientists, plant nutrition
Select Language:
Main Menu
Please click the main subject to get the list of sub-categories
Services offered
  Section: Biotechnology Methods » Enzymology
Please share with your friends:  

Construction of the Maltose Calibration Curve


Maltose is a reducing disaccharide. Maltose reduces the alkaline solution of 3.5 dinitro salicylic acid (DNS), which is pale yellow, into an orange-red complex of 3-amino –5-nitro salicylic acid. The optical density is measured at 540 nm. The intensity of the color depends on the concentration of maltose.


  1. 3,5–dinitrosalicylic acid (DNS): Dissolve 10 gms of DNS in 200 mL of 2N NaOH. To this, add 500 mL of distilled H2O and 300 mL of sodium potassium tartrate and make up the volume to 100 mL with distilled H2O.
  2. Standard maltose solution: 1 mg/mL in distilled H2O.
  3. Distilled H2O.

Pipette out standard maltose solution ranging from 0.0 to 20 mL into test tubes and make up the volume to 2.0 mL with distilled water. The first tube, with 2.0 mL of distilled water, serves as the blank. Add 1 mL of alkaline DNS-reagent to each tube. Keep all the tubes in boiling water bath for exactly 5 minutes and cool to room temperature. Add 4 mL of distilled water to each tubes and read the OD at 540 nm. Plot the graph with maltose along the x-axis and the optical density along the y-axis. Result: The maltose calibration curve is constructed as a straight line passing through the origin.

Copyrights 2012 © | Disclaimer