Incineration

Purpose	To learn the effect of flaming with dry heat
Materials	Nutrient agar plates 24-hour broth culture of Staphylococcus epidermidis Six-day-old culture of Bacillus subtilis

Procedures

1. With your marking pencil, section an agar plate into two parts.
2. Streak the S. epidermidis culture on one-half of the plate. Label this section Control.
3. Sterilize the loop, take another loopful of S. epidermidis culture and sterilize the loop again in the Bunsen burner flame or bacterial incinerator. When the loop is cool, use it to streak the second half of the plate. Label this section Heated.
4. Repeat procedures 1 to 3 with the B. subtilis culture.
5. Incubate the plates at 35°C for 24 hours.

Results
Read for growth (+) or no growth (−) and record.