Oxidase Test

Aim
To test the oxidase-producing microorganisms.

Principle
The oxidase determines whether microbes can oxidize certain aromatic amines, e.g., paraaminodimethylalanine, to form colored end products. This oxidation correlates with the cytochrome oxidase activity of some bacteria, including the genera Pseudomonas and Nisseria. A positive test is important in identifying these genera, and also useful in characterizing the Enterobacteria, which are oxidase negative.

Materials

• Glassware
• Sample
• Culture of Pseudomonas
• Bacillus
• E.coli.
• Staphylococcus aureus
• Klebsiella

Chemicals

• Tetramethyl phenyl diamine
• Dihydrochloride

Procedure

1. Plate method: Separate agar plates streaked with Pseudomonas, Klebsiella, and Bacillus are taken, 1% reagent tetra methyl phenyl diamine hydrochloride was directly added to the plates, and the reaction were observed.

Result

FIGURE 1 EMB.

FIGURE 2 Lactose broth.

FIGURE 3 Mac conkey.

FIGURE 4 Urease.