Vaccines for Foot and Mouth Disease Virus (FMDV)
Vaccines produced from genetically engineered E. coli cells cannot compete with those extracted from virus particle. Therefore, much more work is to be done to make the vaccines available for FMDV on a large scale at low price. An outline for production of FMD vaccine is given in Fig. 5.6.
Synthetic peptides have also been produced and used for immunization against bacterial (Diphtheria, Streptococcus pyogenes) and viral (Hepatitis virus, FMDV) diseases. For the production of synthetic polypeptide to be used as vaccines, it is necessary to have the knowledge of structure and function of proteins and the regions involved in immunogenic response. For example, synthetic polypeptides having immunogenic affects against HBV contain disulphide bond in the region between amino acids 117 and 137 corresponding to the viral surface antigen. After injection into mice the polypeptides elicited antibodies against HBsAg and protected half of mice.
Similarly, a synthetic polypeptide has been identified that corresponds several regions of FMDV protein VP1. The region between amino acids 141 and 160 elicited and production of antibodies against FMDV in guinea pigs, rabbits and swine.