Sterilization of Glassware, Equipments and Culture Media
The thoroughly washed glassware and equipments and carefully prepared culture media are sterilized by heat, steam or millipore filter paper (Table 6.4 ). The sterilized materials are removed when temperature cools down and used according to procedure adopted. For detailed description see In Vitro Culture Techniques: The Biotechnological Principles
Table 6.4. Sterilization of glassware and equipments.
||Apparatus containing glass and silicon tubing, disposable tips for micropipettes, dispensor tubing for pupet, glass bottles with screw caps, magnetic stirrer bars, screw caps, silicon tubing, topper-rubber, silicon, millipore filters, etc.
||Glassware, glass coverslips, glass slides, instruments, Pasteur pipettes, pipettes, test tubes, etc.
(=121°C) for 20 minutes; **160°C/ 1 hour.
Table 6.5. Sterilization of liquid by different methods.
||Agar, antibiotics, EDTA, glucose, glycerol, HEPES, lactalbumin hydrolysate, NaHCO3 solution, NaOH solution, phenol red, salt solution (with glucose), tryptose, water
||Glucose solution, HC1, NaHCO3 solution, NaOH solution,
||Collagenase, antibodies, glutamine, sodium pyruvate solution, transferrin, trypsin, vitamines
||Bovine serum albumin
|Steam (30 min at 100°C)
(=121°C) for 30 minutes, **0.2 mm pore size filter.