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  Section: Plant Lab Protocols
 
 
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Plant Lab Protocols

 
     
 
Methodology for Plant Science

Science grows through the nurturing with appropriate methodologies. Choice of experimental methods in research is important to yield satisfactory results.

 
Subjects like physiology, genetics, pathology, microbiology are now getting expressed in a common language of chemistry, and in common procedures. Quite a number of books are available on general and clinical biochemistry and also on specialized topics such as proteins, nucleic acids, etc. Although there are a number of biochemical procedures followed in plant science research, details are found scattered in various journals and in some science works.
 
There is obviously a need for a source which in one place provides a various biochemical procedures that are in frequent use in agricultural research.

The outcome of our endeavour is the Biocyclopedia.com Plant Lab Protocol covering protocol useful for scientist working in area of plant sciences.

Although we have taken enough care to include operational details, it is necessary for the individual scientist to get conversant with the methods properly.


Biocyclopedia is not respondsible for any kind of inappropriate use of the methodologies given on this website.

   
  Carbohydrates  
   
Color reactions of carbohydrates
Determination of amylose
Determination of fructose and inulin
Determination of glucose by glucose oxidase method
Determination of reducing sugars by Nelson-Somogyi method
Determination of total carbohydrates by anthrone method
Estimation of cellulose
Estimation of crude fiber
Estimation of hemicelluloses
Estimation of pectic substances by:
Colorimetric method
Gravimetric method
Estimation of reducing sugar by dinitrosalicylic acid method
Estimation of starch by anthrone reagent
Phenol suplhuric acid method for total carbohydrate
 
 
 
   
  Lipids  
    Determination of iodine value of oil
Determination of peroxide value
Estimation of free fatty acids
Estimation of oil in oilseeds
Estimation of volatile acids
Identification of quantification of fatty acids
Saponification value
 
 
 
   
  Amino Acids & Proteins  
    Ammonium sulphate fractionation of proteins
Determination of net protein utilization, digestibility and biological value
Electrophoresis of proteins in starch gels
Enzyme-linked immunosorbant assay (ELISA)
Estimation of lysine
Estimation of methionine in food grains
Estimation of proline
Estimation of total free amino acids
Estimation of tryptophan
Fluorography of polyacrylamide gels
Identification of proteins
Immunodiffusion in agrose gels
In vitro translation assay
Isoelectric focusing
Mass Screening test for high-lysine grains
Nitrogen analysis by micro-Kjeldahl method
Polyacrylamide disc gel electrophoresis
Polyacrylamide-sodium dodecyl sulphate slab gel electrophoresis (SDS-PAGE) of proteins
Preparation S-30 extract for protein synthesis in vitro
Production of antiserum
Protein (Western) blotting
Protein digestibility in vitro
Protein efficiency and net protein ratio
Protein estimation – Bradford method
Protein estimation by Lowry’s method
Quantification of proteins in polyacrylamide gels
Rocket immunoelectrophoresis
Sample preparation for amino acids estimation
Silver staining of proteins
 
 
 
   
  Enzymes  
    Acetyl cholinesterase
Aminotransferases
   i) Asparatate amino transferase
   ii) Alanine aminotransferase
Amylases
Ascorbic acid oxidase
Asparagine synthetase
Catalase
Cellulases (C1 and Cx)
   i)   Viscometric method
   ii)  Colorimetric method
   iii) Combined assay
Glutamate dehydrogenase
Glutamate synthase
Glutamine synthetase
Indole acetic acid oxidase
Isoenzyme analysis
   i)   Esterase
   ii)  Polyphenol oxidase (PPO)
   iii) Peroxidase
   iv) Acid phosphatase
   v) Glutamine synthetase
   vi) Phosphoenol pyruvate carboxylase (PEP Case)
   vii) Glutamate dehydrogenase
   viii) Indolylacetic acid oxidase
   ix) Malate dehydrogenase
   x) Sucrose synthase
   xi) Aspartate aminotransferase
   xii) Glutamate synthase
Lipase
Malate dehydrogenase
Nitrate reductase
Nitrite reductase
Papain
Pectin methyl esterase
Peroxidase
Phenylalanine ammonia lyase
Phosphatases
   i)  Acid phosphatase
   ii) Assay using glycerophosphate
Phosphoenol pyruvate carboxylase
Polygalacturonase activity
Polyphenol oxidase
Ribulose biphosphate carboxylase
Sucrose synthase
 
 
 
   
  Nucleic Acids  
   
Cultivation of lambda (l) phage
DNA electrophoresis in agarose gel
Estimation of DNA
Estimation of RNA
Extraction of lambda (l) DNA
Extraction of plant DNA
Extraction of plant total DNA (Rapid method)
Isolation of deoxyribonucleic acid (DNA)
Isolation of messenger RNA by affinity chromatography
Isolation of plasmids
Isolation of ribonucleic acid
Rapid isolation of plasmid DNA (Miniscreen)
Recovery of DNA fragments from agarose gels
Restriction of DNA
Southern blotting
 
 
 
   
  Vitamins  
    Ascorbic acid
   i)  Volumetric method
   ii) Colorimetric analysis
Niacin
Riboflavin
Thiamine
 
 
 
   
  Pigments  
    Carotenes
Chlorophylls
Curcumin
Lycopene
 
 
 
   
  Phenolics  
    Capsaicin
   i)  Colorimetric Method
   ii) Spectrophotometric Method
Chlorogenic acid
Lignins
Phenols
Tannins
   i)  Folin-Denis Method
   ii) Vanillin Hydrochloride Method
 
 
 
   
  Antinutritional Factors  
    Cyanogens
Gossypol
Phytic acid
Trypsin inhibitor
 
 
 
   
  Plant Hormones  
    Ethylene
Indoleacetic acid
 
 
 
   
  Nitrogen Fixation  
    Allantoin and Allantoic acid
Hydrogen evolution
Leghaemoglobin
Nitrogenase
 
 
 
   
  Separation Procedures  
    Analysis of aflatoxins
Column chromatography
Paper Chromatography
Thin layer chromatography
 
 
 
   
  Cell Fractionation  
    Fractionation of cellular components
Isolation of chloroplasts
Isolation of mitochondria
 
 
 
     
 
     
 
 
     









     
 
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