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  Section: Microbiology Methods
 
 
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Microbiology Methods

 
     
 
Basic Techniques of Biotechnologies
Orientation to the Microbiology Laboratory
  The Microscope
  Handling and Examining Cultures
Microscopic Morphology of Microorganisms
  Hanging-Drop and Wet-Mount Oreparations
    Preparing a Hanging Drop
    Preparing a Wet Mount
  Simple Stains
Differential Stains
  Gram Stain
  Acid-Fast Stain
  Special Stains
    Staining Bacterial Endospores (Schaeffer-Fulton Method)
    Flagella and Capsule Stains
Cultivation of Microorganisms
  Culture Media
  Streaking Technique to Obtain Pure Cultures
  Pour-Plate and Subculture Techniques
  Culturing Microorganisms from the Environment


Destructions of Microorganisms
Physical Antimicrobial Agents
  Moist and Dry Heat
    Moist Heat
    Dry Heat
    Incineration
    The Autoclave
Chemical Antimicrobial Agents
  Disinfectants
  Antimicrobial Agent Susceptibility Testing Resistance
    Agar Disk Diffusion Method
    Broth Dilution Method Determining Minimum Inhibitory Concentration
    Bacterial Resistance to Antimicrobial Agents: Enzymatic
    Bacterial Resistance to Antimicrobial Agents: Mutation



Diagnostic Microbiology In Action
Principles of Diagnostic Microbiology
  Primary Media for Isolation of Microorganisms
  Some Metabolic Activities of Bacteria
    Simple Carbohydrate Fermentations
    Starch Hydrolysis
    Production of Indole and Hydrogen Sulfide, and Motility
  Activities of Bacterial Enzymes
    The Activity of Urease
    The Activity of Catalase
    The Activity of Gelatinase
    The Activity of Deoxyribonuclease (Dnase)
    The Activity of a Deaminase
  Principles of Antigen Detection and Nucleic Acid Assays for Detection Identification of Microorganisms
    Antigen Detection Assays
    Enzyme Immunoassay (Eia)
    Nucleic Acid Detection Assays
Microbiology of the Respiratory Tract
  Staphylococci
    Isolation and Identification of Staphylococci
    Staphylococci in the Normal Flora
  Streptococci, Pneumococci, and Enterococci

    Isolation and Identification of Streptococci
    The CAMP Test for Group B Streptococci
    Identification of Pneumococci
    Identification of Enterococci
    Streptococci in the Normal Flora
  Haemophilus, Corynebacteri and Bordetella
    Haemophilus
    Corynebacteria
    Bordetella
  Clinical Specimens from the Respiratory Tract
    Laboratory Diagnosis of a Sore Throat
    Laboratory Diagnosis of Bacterial Pneumonia
    Antimicrobial Susceptibility Test of an Isolate from a Clinical Specimen
Microbiology of the Intestinal Tract
  The Enterobacteriaceae (Enteric Bacilli)
    Identification of Pure Cultures of Enterobacteriaceae from the Normal Intestinal Flora
    Isolation Techniques for Enteric Pathogens
    Identification Techniques for Enteric Pathogens
    Serological Identification of Enteric Organisms
    Techniques to Distinguish Nonfermentative Gram-Negative Bacilli from Enterobacteriaceae
    Rapid Methods for Bacterial Identification
  Clinical Specimens from the Intestinal Tract
    Culturing a Fecal Sample
    Identification of an Unknown Enteric Organism
    Antimicrobial Susceptibility Test of an Enteric Organism
Microbiology of the Urinary and Genital Tracts
  Urine Culture Techniques
    Examination and Qualitative Culture of Voided Urine
    Quantitative Urine Culture
  Neisseria and Spirochetes
    Neisseria
    Spirochetes
Microbial Pathogens Requiring Special Laboratory Techniques
  Anaerobic Bacteria
  Mycobacteria
  Mycoplasmas, Rickettsiae, Chlamydiae, Viruses and Prions
  Fungi: Yeasts and Molds
  Protozoa and Animal Parasites
  Serological Identification of Patient's Antibodies



Applied Sanitary Microbiology
Bacteriological Analysis of Water
Bacteriological Analysis of Milk


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