Regulation at translation level
Information about translational control in eukaryotes is coming from experiments designed to answer following questions regarding the mechanism of operation of translational machinery.
(i) When there are more than one AUG codons in a mRNA molecule, how does the ribosome know, which one to use for initiation of translation ? (ii) What determines the frequency with which a particular mRNA message is read ? (iii) After finishing translation of an open reading frame (ORF), can a ribosome start again, if an AUG codon follows in the same mRNA within a reasonable length ? (iv) Can a mRNA remain untranslated in a cell and translated only when necessary ?