Algae, Tree, Herbs, Bush, Shrub, Grasses, Vines, Fern, Moss, Spermatophyta, Bryophyta, Fern Ally, Flower, Photosynthesis, Eukaryote, Prokaryote, carbohydrate, vitamins, amino acids, botany, lipids, proteins, cell, cell wall, biotechnology, metabolities, enzymes, agriculture, horticulture, agronomy, bryology, plaleobotany, phytochemistry, enthnobotany, anatomy, ecology, plant breeding, ecology, genetics, chlorophyll, chloroplast, gymnosperms, sporophytes, spores, seed, pollination, pollen, agriculture, horticulture, taxanomy, fungi, molecular biology, biochemistry, bioinfomatics, microbiology, fertilizers, insecticides, pesticides, herbicides, plant growth regulators, medicinal plants, herbal medicines, chemistry, cytogenetics, bryology, ethnobotany, plant pathology, methodolgy, research institutes, scientific journals, companies, farmer, scientists, plant nutrition
Select Language:
Main Menu
Please click the main subject to get the list of sub-categories
Services offered
  Section: Practical Skills in Chemistry » Instrumental techniques
Please share with your friends:  

Phosphorescence and luminescence

Instrumental techniques
  Basic spectroscopy
    Introduction to spectroscopy
    UV Ivisible spectrophotometry
    Fluorescence spectrophotometry
    Phosphorescence and luminescence
    Atomic spectroscopy
  Atomic spectroscopy
    Atomic Absorption Spectroscopy
    Atomic Emission Spectroscopy
    Inductively coupled plasma
    Decomposition techniques for solid inorganic samples
  Infrared spectroscopy
  Nuclear magnetic resonance spectrometry
    1H-NMR spectra
    13C-NMR spectra
  Mass spectrometry
    Interfacing mass spectrometry
  Chromatography ~ introduction
    The chromatogram
  Gas and liquid chromatography
    Gas chromatography
    Liquid chromatography
    High-performance liquid chromatography
    Interpreting chromatograms
    Optimizing chromatographic separations
    Quantitative analysis
    The supporting medium
    Capillary electrophoresis
    Capillary zone electrophoresis (CZE)
    Micellar electrokinetic chromatography (MEKC)
  Electroanalytical techniques
    Potentiometry and ion-selective electrodes
    Voltammetric methods
    Oxygen electrodes
    Coulometric methods
    Cyclic voltammetry
  Radioactive isotopes and their uses
    Radioactive decay
    Measuring radioactivity
    Chemical applications for radioactive isotopes
    Working practices when using radioactive isotopes
  Thermal analysis

A phenomenon related to fluorescence is phosphorescence, which is the emission of light following intersystem crossing between electron orbitals (e.g. between excited singlet and triplet states). Light emission in phosphorescence usually continues after the exciting energy is no longer applied and, since more energy is lost in intersystem crossing, the emission wavelengths are generally longer than with fluorescence. Phosphorescence has limited applications in chemical sciences.

Luminescence (or chemiluminescence) is another phenomenon in which light is emitted, but here the energy for the initial excitation of electrons is provided by a chemical reaction rather than by electromagnetic radiation. An example is the action of the enzyme luciferase, extracted from fireflies, which catalyses the following reaction:

⇒ Equation [26.6] luciferin + ATP + O2 → oxyluciferin + AMP + PPi + CO2 + light

The light produced is either yellow-green (560nm) or red (620nm). This system can be used in biomolecular analysis of ATP, e.g. to determine ATP concentration in a biological sample. Measurement can be performed using the photomultiplier tubes of a scintillation counter to detect the emitted light, with calibration of the output using a series of standards of known ATP content.

Copyrights 2012 © | Disclaimer