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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Study of Drumsticks in the Neutrophils of Females

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

Study of drumsticks in neutrophils of females.

Introduction
The sex chromatin of polymorphic nuclear neutrophils of human blood contains a specific “drumstick”-like nuclear appendages that has its head about 1.5 mm in diameter attached to the nucleus by a threadlike stalk. The drumstick differs from the sex chromatin of other cells by being extruded from the nucleus. It is visible in only a relatively small portion of cells (in about 1/40 neutrophils of a normal female).

Materials
  • Female blood sample
  • Slides
  • Needles
  • Cover slip
  • Alcohol
  • Cotton
  • Leishmann stain
  • Microscope
Procedure
  1. Clean the fingertip with cotton soaked in alcohol and prick it with a sterilized needle.
  2. Place 1 or 2 drops of blood on the right side of the slide.
  3. With the help of another clean slide, smear the blood along the slide, such that a tongue-shaped thin-layered smear was formed and air-dried.
  4. Fix the dried smear with acetone-free methanol or absolute alcohol for about 5 minutes.
  5. Dry and stain the smear with Leishmann stain.
  6. Add distilled water, about double the amount of the stain, on the smear.
  7. Mix the smear using a pipette for 10–20 minutes.
  8. Keep the slide in running water to remove excess stain, and then air-dry.
  9. Observe the slide under the microscope using an oil immersion lens.
 
     
 
 
     




     
 
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