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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Mounting of Genitalia in Drosophila Melanogaster

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

Description
The genital plate is located in the abdominal region of the male and female flies, but in the males the genital plate is more prominent and used as a copulatory pad. It is also called the epandrium. The genital plate is horseshoe-shaped, and it is bent in the posterior region. It is divided into 2 parts, the heel and the toe.

Inside the arch, a pair of anal plates and a pair of primary claspersis are present. On the primary claspers, 6–19 dark spines, or bristles are present, which are very thick. Their number is species-specific. The main function of the genital plate is to hold the female to transfer the sperm into her genital organ during copulation.

Materials
  • Male flies
  • 1N HCl
  • Cresosote solution
  • Cavity slides
  • Glycerine
  • Cover slips
Procedure
  1. Remove the last abdominal segment of the male Drosophila melanogaster.
  2. Transfer the segment into 1N HCl taken in a cavity slide and allow it to sit for 15 minutes.
  3. Blot out the HCl, use 2 or 3 drops of cresote solution, and allow it to sit for about 20 minutes.
  4. Remove the pellicle organ and clean the genital plate.
  5. Transfer the genital plate onto a clean plain slide and mount the genital plate with glycerine
 
     
 
 
     




     
 
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