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  Section: General Biotechnology / Animal Biotechnology
 
 
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Animal Cell, Tissue and Organ Culture

 
     
 
Content
Requirements for animal cell, tissue and organ culture
  Substrates for cell culture
  Substrate treatment
  Culture media
    Natural media
    Synthetic media
  Sterilization of glassware, equipments and culture media
  Isolation of animal material (tissue)
    Disaggregation of tissue
    Establishment of cell culture
Cultivation of animal cell en masse in bioreactor
Immobilized cell culture
Insect cell culture
Somatic cell culture
Organ culture
  Organ culture on plasma clots
  Organ culture on agar
  Organ culture in liquid medium
  Whole embryo culture
Valuable products from cell cultures
  Monoclonal antibodies
  Production of commercial products from insect culture

Substrate Treatment
To increase the efficiency of cultured cells through increased cell attachment, the surface of the substrates is treated with purified fibronectm or collagen (or gelatin or poly D-lysine). This chemical is poured onto the surface of dish (substrate), excess amount drained, chemical is dried and sterilized by using UV light. In addition, the surface of substrates is also treated with monolayer of special* types of cells which is called feeder layer because it feeds the growing cells. The special cells used as feeder layer are glial cells, normal foetal intestine, mouse embryo fibroblast, etc. The feeder layer is used for the growth of neurons, epithelium of breast and coelome, and production of transgenic animals.
 
     
 
 
     



     
 
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